Tiger King’s Carol Baskin’s debut was sparked by an ad shown during the break on Dancing with the Stars.
It was paid for and featured on the family of the missing Netflix star’s ex-husband Don Lewis.
He asked the audience to provide information about her disappearance or Carol Baskin’s alleged involvement.
In the Tiger King series, it was claimed that he fed her to the tigers during the Big Cat rescue – something like that. He has denied Sometimes.
Carol Baskin was given the opportunity to appear on ABC’s Dance with the Stars after a documentary success earlier this year.
Earlier Monday night, she performed a side double with pro dancer Pasha Pashkov, who was caged with the tigers she bought.
It was given 11 by the judges but during the commercial break it was the ad that got the biggest response online.
It included Don Lewis’ three daughters – Gail, Linda and Donna, as well as his former assistant Ann McQueen.
In the short film, the eldest daughter Donna says: “We need to know what happened to our father.”
Family lawyer John Phillips also speaks and specifically names Carol Baskin.
“Don Lewis mysteriously disappeared in 1997. His family deserves answers, they deserve justice. Do you know who did that or if Carol Baskin was involved?” He asks.
The family then offers a reward of up to 100,000 (000 78,000) for information and asks viewers to call a phone line.
Don Lewis went missing 23 years ago. He disappeared a day before a scheduled trip to Costa Rica, and was legally pronounced dead in 2002.
He and Baskin started an animal sanctuary in Tampa, Florida, which later became the Big Cat Rescue Corporation. They were married at the time of his disappearance, but he had filed a restraining order against her two months ago.
Theories about what happened to him became part of the hit Netflix series, including suggestions that Baskin, who acquired his 6 million (£ 4.5 million) fortune, was responsible for his disappearance.
He has vehemently denied any involvement. “Unnecessary lies are better for getting an audience,” he said.